T4 DNA Polymerase
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. Source: Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid. Appl…
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DNA purification
It is easy to use, the protocol is simple and quick. DNA purification can be used in a wide area of research, could led to finding potential protein that is coded from DNA.
Review Date: 7 Oct 2013 | New England Biolabs Inc.
leukemia genetics
I use this for sub cloning PCR product. It's always reliable and powerful enough to get enough yield. Efficiency seems more time-dependent, sometimes I get low yield when I incubate just for a short time < 1hr.
Review Date: 6 Aug 2013 | New England Biolabs Inc.
DNA cloning
Excellent consistency and long term quality. This product works well with NEB's other products - I would recommend their entire line of enzymes to my colleagues.
Review Date: 3 Jun 2013 | New England Biolabs Inc.
ligation
Excellent product and great website. Company provides great service. Works well. Good value for money. Highly recommended. I have been using it for a while and it works every time.
Review Date: 3 Jun 2013 | New England Biolabs Inc.
Not good.
Review Date: 5 Jan 2011 | New England Biolabs Inc.
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.
Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid.
Applications:
3´ overhang removal to form blunt ends (1,2).
5´overhang fill-in to form blunt ends (1,2).
Single strand deletion subcloning (3).
Second strand synthesis in site-directed mutagenesis (4).
Probe labeling using replacement synthesis (1,2).
