Refine™ 96-Well Ultra-Filtration Plate
UCT's Refine TM Ultra-Filtration technology allows for sample precipitation and filtration to occur simultaneously in the same well plate or column.
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Refine™ Ultra-Filtration technology enables a fast, and easy-to-follow protocol in under 15 minutes. The novel, hydrophobically treated, dual frit combo mitigates any leakage of the sample/organic solvent through the plate before the application of vacuum. This ensures adequate filtering rather than pelleting precipitated proteins, not only extending the lifetime of an analytical column, but also eliminating any sample transfer steps.
- Can be used with a wide range of biological sample types, such as plasma and urine
- Accommodates in-well urinary hydrolysis and can withstand high incubation temperatures
- Provides additional clean-up and filtration for cannabis analysis
- Processes 96 samples in less than 20 minutes when using the wellplate
- Long-drip tips on wellplate reduce sample errors and risk of inter-well contamination and cross-talk
- Works on all current manual or automated processing instrumentation
In-well hydrolysis using UCT Refine Ultra-Filtration Technology
In this application note, UCT outlines a suggested procedure for in-well hydrolysis of urine samples using the Refine™ Ultra-Filtration Technology. Various biological sample types commonly contain proteins that complicate downstream analysis if not effectively removed. While sample preparation techniques, such as solid phase extraction (SPE), can successfully remove these unwanted matrix components, complex and lengthy extraction procedures are not ideal for high-throughput laboratories. With the advancement of LC-MS/MS technology, many laboratories have implemented dilute and shoot procedures in order to reduce sample preparation time, reduce consumable spending and reduce overall turnaround time. The caveat to this quick protocol is the toll that it ultimately takes on the HPLC column and mass spec source.
The Refine Ultra-Filtration Technology allows for sample precipitation and filtration to occur simultaneously in the individual column/well. A novel, hydrophobically treated, submicron frit combination facilitates the removal of sample proteins without a complicated extraction. Using the Refine Ultra-Filtration Technology reduces column backpressure while also reducing analyte suppression when compared to a standard dilute and shoot protocol.
