Ready-to-Use Lentiviral Packaging Plasmid Mix
Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors. The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to…
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Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors.
The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to produce pseudotyped packaged lentiviral expression constructs when co-transfected with an HIV-based lentiviral expression construct (e.g. Cellecta pRSI shRNA expression constructs) into a producer 293T cell line (e.g. ATCC, Cat.# CRL-11268™). Pseudotyped lentiviral particles with VSV-G envelope protein can infect a variety of mammalian or non-mammalian, dividing or non-dividing cells. The kit contains 250 µg of lentiviral packaging plasmid mix that will be sufficient for 25 transfections in 10-cm culture plates.
Cellecta’s packaging mix contains tested anion-exchange purified packaging plasmids pre-mixed at the proper ratio for optimal production of packaged VSV-g pseudotyped lentiviral particles. This reagent is used in conjunction with HEK 293T cells.
The Ready-to-Use Lentiviral Packaging Plasmid Mix is compatible with most commercially-available second- and third-generation lentiviral vectors.
Development of Recombinant Protein Overproducer Cell Lines with Lentiviral Expression Vectors
Custom overproducer cell line services are offered using the developed protocols, for the development of a wide range of cell lines overproducing recombinant proteins. Using Cellecta’s lentiviral system, a construct containing the gene of interest can be packaged into VSV-g pseudotyped viral particles and delivered into a wide range of mammalian cells. With an average development time of 6 weeks, these cell lines dramatically improve the efficiency of process development and can be used for preparative and industrial-scale production of proteins.
Modified sgRNA Design Improves Results of CRISPR Knockout Screens
Optimization of the sgRNA design used in CRISPR knockout screens has been the goal of several research groups in recent years. This application note aims to address points raised in recent studies, including whether modifications of the 80 bases downstream of the target region can increase the rate or knock-out efficiency of the active CRISPR system. Showing that it is possible to increase the quality of sgRNA libraries, with just a few changes to the 3' region.
