Perfecta3D Hanging Drop Plates - 96-Well

The data shown in this application note illustrate how easy it is to generate uniform spheroids, treat with test compounds, and obtain sensitive viability and cytotoxicity results, even from a single set of spheroids, using the Perfecta3D Hanging Drop Plates and Cayman assays optimized for 3D cell culture.

This case study describes how scientists at the Huntington Medical Research Institutes are using 96- and 384-well Perfecta3D Hanging Drop Plates to form models of human tumors using appropriate co-cultures of carcinoma cell lines and fibroblasts.

Tumor Histoids (TH) are living three‐dimensional human mini‐tumors consisting of stroma invaded by tumor cells. They can be produced in quantity and format suitable for high throughput screening. Stromal‐epithelial interactions occur spontaneously during TH culture and model those that activate pathways controlling tumor growth, malignant progression and response to therapies in vivo. This report describes the adaptation of the original TH production method to production in Perfecta™ hanging drop plates, as well as an experiment comparing cisplatin effect on TH, tumor cell spheroids and monolayers.

3D cell culture systems are essential to acquiring physiologically‐relevant results. However, scaling up and maintaining reproducibility of 3D cell cultures have been difficult due to lack of standardized tools and methods that are simple to use and compatible with existing equipment. This scientific poster present a novel 3D cell culture platform that enables uniformly-sized spheroids to be generated and tested in a 384-microplate format and using high throughput screening instruments, such as plate readers, liquid handling robots, and automated imaging and analysis systems.

This white paper illustrates the importance of three-dimensional (3D) cell culture in the production of pluripotent stem cell (PSC)-derived models of embryo development and differentiation. It contains a general overview of embryoid bodies (EBs) and a comparison between the various methods used for their generation. There is also a discussion about using teratoma assays versus EBs to validate pluripotency with newly derived PSC lines. Finally, there is a brief description of recent publications illustrating how 3D culture can be used to maximize differentiation.

To obtain more physiologically relevant data, researchers have developed various 3D cell culture techniques to replicate the in vivo characteristics of physiological tissues. This white paper reviews the current methods and systems that are available for spheroid culture. In addition the benefits and applications of the Perfecta3D™ Hanging Drop Plates from 3D Biomatrix are discussed. 3D cell culture combined with high-throughput screening is likely to accelerate pharmaceutical development.

New testing methods are imperative at early stages of drug development to reduce the expensive, late-stage failures due to efficacy and safety. Drug testing data, particularly in oncology, from in vivo-mimicking 3D cell cultures have the potential to play a pivotal role in early-stage decision-making about the probability of therapeutic candidates’ success. This white paper demonstrates the capabilities of the Perfecta3D Hanging Drop Plates from 3D Biomatrix for spheroid growth and subsequent high-throughput testing of anti-cancer compounds at the preclinical discovery phase.

Watch this video to learn more about 3D Biomatrix’s Perfecta3D® Hanging Drop Plates, which enable uniformly-sized spheroids to be generated and tested in a multi-well microplate format. Laura Schrader, CEO at 3D Biomatrix, explains how the hanging drop method works and the key benefits of the system. Laura also discusses how such systems may enable drugs to get to the market in a much quicker time and at a much lower cost.