NutriStem® hPSC XF Medium
Defined, xeno-free, serum-free medium, Designed for optimal growth and expansion of human iPS and hES cells.
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NutriStem® hPSC XF Medium is a widely published, defined, xeno-free, serum-free cell culture medium designed to support the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells.
Protocols have been established around the world for applications ranging from derivation to differentiation. NutriStem® hPSC XF Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, superior cell attachment and proliferation is observed with NutriStem® hPSC XF Medium aid high-throughput screening applications. NutriStem® hPSC XF Medium exhibits a consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation as well as increased reproducibility shown in long-term growth of over 50 passages.
Features
- Defined, serum-free, and xeno-free
- Complete, ready-to-use formulation (no additional supplements required)
- Drug Master File (DMF) available, produced under cGMP
- Low-protein formulation
- Contains stable L-alanyl-L-glutamine and HSA
- Enables efficient expansion and growth of hES and hiPS cells in feeder-free culture systems
- Cells maintain pluripotency, normal phenotype (colony morphology), and normal genotype (karyotype) through long-term culture (50+ passages)
- Extensively tested and widely used on multiple hES and iPS cell lines, including H1, H9.2, I6, I3.2, and CL1
Assuring Pluripotency of ESC and iPSC Lines
Compared to other cell types, stem cells have the unique ability to “self-renew” their populations by dividing into identical daughter stem cells as well as a capacity for “differentiation” into more specialized cell types. In this way, stem cells provide the body’s foundation during embryogenesis and facilitate tissue growth and repair later in life. However, stem cells demonstrate different degrees of differentiation potency. Pluripotent stem cells are of particular interest in clinical and developmental biology research as these cells have the capacity to specialize into cell types originating from the three germ layers (i.e., ectoderm, mesoderm, and endoderm) that go on to form the entire body.
