LightCycler ® 2.0 Instrument
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in…
Nice design, reproducible results, high sensitivity
Gene analysis
Once you get used to the equipment, its design becomes easy to use. Consistent results, high responsiveness, and an intuitive, easy-to-use platform.
Review Date: 20 May 2022
PCR
I've used the carousel based PCR system extensively for about a year. The set up of reagents are easy and for the most part pretty fast. We used 20ul reactions and our runs on averagely took us about 35 minutes. The biggest drawback to this system was that the capillaries you load your sample in are glass. Occasionally one would break due to miss handling or just weak glass. Setting up a new run wasn't a problem but we would have to bleach everything down to prevent contamination. Other than that I really enjoy running PCR on this product.
Review Date: 17 Jan 2014
Review Date: 15 Jul 2011
Very easy to use and great technical support, however no good for large treatment groups due to size limitations.
Review Date: 24 Nov 2010
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries. Their superior surface-to-volume ratio provides extremely rapid thermal transfer, which facilitates high-speed thermal cycling. The combination of LightCycler® Capillaries and the air-driven temperature control of the LightCycler® 2.0 Instrument ensures rapid PCR. An entire 35-cycle run can be performed in as little as 30 minutes (with 20 μl capillaries) to 60 minutes (with 100 μl capillaries).
Fluorescence detection formats: The LightCycler® 2.0 Instrument is optimized for two fluorescence detection formats: SYBR Green I, HybProbe probes, and hydrolysis probes. In addition, the instrument supports a wide variety of other fluorescence detection formats, such as monocolor SimpleProbe probes and other formats based on FRET (fluorescence resonance energy transfer). However, it is essential that the fluorescent dyes used are compatible with the LightCycler® 2.0 optical unit.
Software: The LightCycler® 2.0 Instrument utilizes the LightCycler® Software 4.1, an optimized version of the LightCycler® 4.0 and 4.05 Software, which provides advanced data-analysis modules, efficient data management, and effective data protection.
Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis
Promoter hypermethylation is a frequent mechanism for the repression of gene transcription in cancer and is regarded as one of the hallmarks of cancer. Analysis of DNA methylation is a promising tool for early cancer detection, risk assessment and response to therapy. The most popular approaches for DNA methylation detection rely on the treatment of genomic DNA with sodium bisulfite. Several simpler PCR-based methods have also been developed which are especially important for small scale research labs. Download this application note to read about is high-resolution melting analysis (HRM), based on the “melting” properties of DNA in solution.
