ISOLUTE® PLD+ Protein and Phospholipid Removal Plates
ISOLUTE® PLD+ Plates and Columns streamline LC-MS/MS sample preparation by efficiently removing proteins and phospholipids from plasma and blood samples in just three simple steps, ensuring high analyte recovery, enhanced sensitivity, and smooth processing with vacuum, positive pressure, or automated systems.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Bioanalysis
This is an excellent extraction plate for removing phospholipids from plasma samples. These kinds of plates PLD+ can really compete with Waters Ostro plates.
Review Date: 18 Feb 2014 | Biotage
ISOLUTE® PLD+ Protein and Phospholipid Removal Plates and Columns provide a very effective but extremely simple sample clean-up for LC-MS/MS analysis. Using a simple solvent crash/filtration-based procedure, proteins and phospholipids are simultaneously removed from plasma and other blood-based samples while high, reproducible analyte recoveries are maintained.
Benefits:
- Excellent protein and phospholipid removal leads to enhanced sensitivity and better analyte recovery.
- Effective sample clean-up in 3 simple steps without compromising analyte recovery.
- Excellent flow characteristics allowing for trouble-free processing using either positive pressure or vacuum based system as well as automated liquid handlers.
Extraction of vitamins B1 and B6 from whole blood using ISOLUTE PLD+ Protein and Phospholipid Removal Plate
Vitamin B1 (Thiamine Diphosphate, TDP) and B6 (Pyridoxal-5- Phosphate, PLP) in whole blood is measured clinically to help measure various metabolic functions. TDP and PLP are highly water soluble (logP= -5.9, -2.2 respectively) and are extremely light-sensitive. Traditionally, TDP and PLP are measured through fluorescence detection, requiring the use of hazardous reagents for derivatization. This additional derivatization step is also time consuming. In this application note, Biotage describes a simple ISOLUTE® PLD+ protocol for the extraction of vitamin B1 and B6 (TDP/PLP) from whole blood and LC-MS/MS analysis. High reproducible recoveries and low protein and phospholipid coextraction are demonstrated. ISOLUTE PLD+ Protein and Phospholipid Removal plates and columns offer a fast and extremely simple sample preparation approach. Proteins are efficiently removed from the sample using an in-plate, ‘solvent first’, protein crash and filter procedure, which is easily automated.
ISOLUTE PLD+ Protein and Phospholipid Removal Plates
In this video, Biotage presents ISOLUTE® PLD+ Protein and Phospholipid Removal Plates. ISOLUTE PLD+ Protein and Phospholipid Removal Plates provide a very effective but extremely simple sample clean-up for LC-MS/MS analysis.
