Invitrogen™ Attune™ Flow Cytometer - Blue/Red Lasers
Blue (488nm), Red (637nm) Excitation Lasers.
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If I could exchange it for a better flow from a company with great service, I would
Flow cytometry, cell cycle progression, cell death, pulse chase
I have not had a great experience with this equipment. We purchased it around 5 years ago and I have not been able to get successful results in any experiment. Customer care is ambiguous in their responses, where depending who you call you get different answers and none work. Some of their own protocols do not work well, thus I am disappointed with this equipment.
Review Date: 19 Oct 2016 | Thermo Fisher Scientific
Manufacturer's Response
“We are currently in communication with Dr. Martinez-Montemayor in an attempt to resolve the handling issues that she has been experiencing with her first generation Attune Flow Cytometer. We take pride at Thermo Fisher Scientific in the quality of our products and services, and we offer a wide range of additional training options to ensure that our customers can get the most out of our products.<br><br> Since the release of the Attune NxT Flow Cytometer in 2014, we have made significant investments around the globe in service and support personnel and training. With this, we are confident that we offer an exceptional experience for anyone using our Attune or Attune NxT flow cytometer.”
Modular design, up to 4 lasers and 14 colors.
The Invitrogen™ Attune™ NxT flow cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match the panels you are currently running. Multiple fluorescent proteins can be detected with an optional 561 nm laser. Whether you configure your system now or upgrade later, the Attune NxT flow cytometer can grow with you and your research needs.
This powerful, flexible flow cytometer offers:
- Modular design—Multiple configurations available—field upgradable.
- Save time—10X faster speeds with no loss in data quality.
- Simplified sample prep—No wash, no lyse options, non-clogging fluidics.
- Enables unique applications—Complex protocols on a broad range of cell types and samples.
Flow Cytometry Analysis of Transcription Factor Expression During Differentiation of hPSC-Derived Cardiomyocytes
The ability to direct human pluripotent stem cells (hPSCs) toward differentiated cell phenotypes offers tremendous potential for personalized and regenerative medicine. The identification of key transcriptional regulators of pluripotency, as well as chemically defined media and cell culture conditions that drive PSCs towards distinct cell fates, have enabled researchers to derive a multitude of differentiated cell types with a high degree of control and precision. In this application note the Attune Nxt Flow Cytometer is demonstrated to enable single-cell quantification of cells expressing markers of both pluripotency and cardiomyocyte specification.
Ten-Parameter Immunophenotyping of Human Lysed Whole Blood with the Attune NxT Flow Cytometer Allows Identification of Multiple T-Cell Subsets and Myeloid Cells
Immunophenotyping is the analysis of heterogeneous populations of cells within human blood for the purpose of identifying the presence and proportions of various cell populations. Multiparameter flow cytometry is a widely-used method for identifying cell subsets within complex cellular systems. This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained human whole blood using a stain-and-lyse protocol.
Acoustic Flow Cytometry for Tumor and Liquid Biopsy Identification & Isolation
Watch this video to find out how the Autónoma University of Madrid is using the Invitrogen™ Attune™ NxT Flow Cytometer for acoustic-based flow cytometry. This technology enables reliable and efficient identification and isolation of cells from tumors and liquid biopsies.
