Glycomics
Stable Isotope-labeled Standards for Glycan Research
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The emerging field of glycomics focuses on the structure and function of glycans in a cell, tissue, organism, or other living system. Glycans are chained carbohydrates (oligo or polysaccharides) that are free or conjugated to macromolecules, such as proteins (referred to as glycoproteins) or lipids (referred to as glycolipids). They participate in a diverse array of biological processes (e.g., cell signaling, protein folding, immune recognition) and are implicated in a number of diseases (e.g., oncological, autoimmune, Alzheimer's). While analytical methodologies for their detection and sequencing have advanced, glycomics research has been hampered by the lack of commercially available standards and research tools.
Individuality normalization when labeling with isotopic glycan hydrazide tags (INLIGHT): A novel N-linked glycan relative quantification strategy
Bioanalytical characterization and quantification of protein post-translation modifications (PTMs) is fundamental to elucidating the functions and activities of PTMs in mediating intra- and extra-cellular interactions. In this application note, Cambridge Isotope Laboratories describes how incorporation of a stable-isotope label allows for relative quantification of N-linked glycomes derived from two different samples.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
Metabolic incorporation of stable isotope labels into glycans
In this application note, Cambridge Isotope Laboratories presents an in vivo labeling strategy for glycomic studies, analogous to the SILAC approach used for proteomics. This glycomic methodology is called IDAWG – isotopic detection of aminosugars with glutamine – and is based on the side-chain of glutamine being the sole nitrogen donor source in the biosynthesis of aminosugar nucleotides.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
