Flow Cytometry Kits
R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specific…
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R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies.
Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor the presence of cytokines and cytokine receptors as well as Fluorokine® kits; labeled cytokines that can be used to monitor the presence of cytokine receptors.
Features of the Flow Cytometry Kit:
• Simultaneous detection multiple different targets
• Rapid & efficient
• Single step staining
R&D Fluorokine Receptor Detection Kits employ recombinant biotinylated ligands to quantitatively determine cytokine receptor density using flow cytometry. These simple kits allow the user to estimate the percentage of cells within a population which express the receptor of interest. In addition, cell surface receptor density can be assessed.
Flow Cytometry Kit Contents (Multi-Color):
• Fluorochrome conjugated antibodies
• Isotype controls
• All necessary buffers
Flow Cytometry Kit Contents (Fluorokine Receptor):
• Biotinylated recombinant, species specific ligand
• Avidin conjugated with fluorescein (at an f:p ratio of 5:1)
• Negative control reagents
• Blocking antibody
• Wash buffer concentrate
Multi-Color Flow Cytometry Protocol to Study Mesenchymal Stem Cells within a Heterogeneous Population
Mesenchymal stem cells (MSCs) are a rare population with an incompletely defined immunophenotype. In order to study MSCs, it is necessary to validate the maintenance of undifferentiated cells in culture and analyze the lineage commitment of mesenchymal stem cells under specific experimental conditions.
