CyGEL Sustain
A novel thermoreversible gel mountant for extended immobilization and imaging.
CyGEL Sustain pack
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Biomedicine/ Zebrafish/ Drug Screening
CyGel Sustain was our miracle solution to perform the extremely technically challenging intraocular injections in zebrafish larvae at 2 days post fertilization, in a 100% accurate and reproducible way. Thanks to CyGel Sustain we could immobilise these larvae (no more than 2 mm long) and carry out the intraocular injections into their eyes (no more than 50 microns diameter) under the dissecting microscope. After injection, CyGel was reverted to liquid and larvae recovered with no damage (Reference(s): Alvarez, Y., et al. PLoS One 4.11 (2009): e7867.) We have used CyGel Sustain since then for different protocols where safe and reversible immobilization of the small larvae is needed with excellent results. Biostatus outstanding support and contribution for the refinement of our protocols and overcome of technical difficulties, is not easy to find among the post-sale service of other companies.
Review Date: 4 Jun 2014 | Biostatus Limited
CyGEL Sustain is a novel thermoreversible gel that is compatible with extended imaging of LIVE cells, for several hours depending on cell type.
Its special formulation is optimised for the addition of RPMI and similar culture media.
It can be used to immobilise non-adherent cells and beads by simple warming, and conversely allowing their recovery by simple cooling. CyGEL Sustain is unusual in that it is a liquid when cold and a gel when warmed!
CyGEL Sustain has many applications in imaging, flow cytometry and cell-based screening. It is compatible with LIVE cells, usefully allowing their immobilisation for analysis and subsequent release for ongoing experimentation.
Key benefits include:-
- Optically clear with low autofluorescence
- Controllable and rapid transition from liquid to gel
- Convenient immobilisation of non-adherent cells and beads
- Compatible with GFP and "in gel" fluorescent probes including DRAQ5™
CyGEL Sustain™ in Image-Based Apoptosis Assays: Extended Immobilization of Non-Adherent / De-Adhering Cells
Microscopy offers the ability to gain important morphological information, including nuclear appearance to identify apoptotic events, or even subcellular location of other markers of interest. Cells undergoing death processes modify both localization and mobility properties of DNA-binding proteins and other compartmentalized and membrane‐located proteins allowing stages of apoptosis to be resolved. Sometimes there is a need to immobilize cells or even whole organisms over several hours to analyze events or signals of interest. This application note demonstrates how CyGEL Sustain™ provides a long-acting thermoreversible hydrogel mountant for non-adherent/motile cells.
