CRISPRtest™ Functional Cas9 Activity Kit
Want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors, the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA), and the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA. Transduction of CRI…
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Want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors,
- the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA), and
- the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA.
Transduction of CRISPRtest virus into cells will result in a mixture of cells expressing GFP and cells expressing RFP. Upon cell growth in the presence of active Cas9, the ratio of GFP cells: RFP cells will decrease proportionally to Cas9 activity.
The CRISPRtest Functional Cas9 Activity Kit, currently for human cell lines only, is an easy-to-use, highly sensitive kit for measuring functional Cas9 activity in almost any human cell line.
Brochures
Cellecta: Your Gene Knockout Solutions Partner
Discover Cellecta's CRISPR knockout portfolio in this brochure, including constructs, polled lentiviral libraries, screening service and customer cell lines.
CRISPR/Cas9 Genome-Wide gRNA Library for Target Identification
Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological responses, and complex pooled lentiviral-based libraries expressing large numbers of genetic disruptors, such as shRNAs, make large-scale cell screening practical. This study investigates the potential differences in the RNAi and CRISPR HTGS technologies, by performing parallel dropout viability screens using module-1 human genome-wide CRISPR sgRNA library and module-1 human genome-wide RNAi shRNA library, both modules targeting the same set of 6,300 genes with the same number of effectors (sgRNA or shRNA) for each gene.
Screening for Genes Regulating TGF-β-Induced Apoptosis
TGF-β signaling has been extensively studied, and as such provided a good model system to assess the performance of Cellecta’s shRNA screening library. TGF-β, a multifunctional extracellular cytokine, inhibits cell proliferation by arresting cell cycle progression and inducing apoptosis; however, it can also promote metastasis and angiogenesis in some cancer cells. This acquired resistance to the growth-inhibitory function suggests disruption of the TGF-β apoptotic mechanism by genetic alternations that inactivate the signal transduction genes in the pathway.
Modified sgRNA Design Improves Results of CRISPR Knockout Screens
Optimization of the sgRNA design used in CRISPR knockout screens has been the goal of several research groups in recent years. This application note aims to address points raised in recent studies, including whether modifications of the 80 bases downstream of the target region can increase the rate or knock-out efficiency of the active CRISPR system. Showing that it is possible to increase the quality of sgRNA libraries, with just a few changes to the 3' region.
Optimizing CRISPR/Cas9 for Easy Knock Out, Activation or Repression of Genes
The CRISPR/Cas9 technology, easy to adapt and use, has become the latest gene editing go-to for life scientists. From the design of guide RNA to the expression of different forms of Cas9, Dr. Sylvain Baron, research scientist at Cellecta, shares his projects in optimizing several aspects of CRISPR to make it much easier to knock out, activate or repress genes.
