CellScape™ Precise Spatial Proteomics (PSP) Platform

VistaPlex assay kits are modular, ready-to-use multiplex immunofluorescence (mIF) assays designed to deliver comprehensive cellular phenotyping and spatial tissue insights for advanced spatial biology applications. Enhanced by EpicIF technology, the CellScape Precise Spatial Proteomics platform now supports expanded multiplex dye compatibility, ensuring that next-generation VistaPlex kits integrate seamlessly with the EpicIF workflow. Data presented highlight the effectiveness of the Segmentation, Spatial Immune Profiling, and Architecture panels across a wide array of human FFPE tissues, revealing distinct biological insights achievable with individual kits or combined panel configurations.

VistaPlex Spatial Immune and Architecture Kits for spatial analysis on human formaldehyde-fixed, paraffin-embedded (FFPE) tissues enable the profiling of immune populations and tissue architecture using validated antibodies and optimized staining protocols. Canopy Biosciences details a study which compares the efficiency of different assay kits and highlights their ability to identify various cellular phenotypes across different tissue types and disease states. Further, Canopy Biosciences emphasizes the compatibility of VistaPlex kits with CellScape Whole-Slide Imaging Chambers, enhancing the reproducibility and utility of the assays in basic and translational research settings. Overall, VistaPlex kits offer a robust and cost-effective solution for spatial biology research, facilitating deep phenotyping of immune and cancer cells in diverse tissues.

Immunohistochemistry is the most widely used diagnostic technique in tissue pathology. However, IHC is associated with several limitations including the labeling of just a few markers per tissue section and limited quantification of cell populations. As a result of plex limitations, key insights about tumor biology are missed, which could be important for advancing our understanding of tumor biology and ultimately improving patient outcomes. ChipCytometry™ is a novel image-based platform for precise spatial multiplexing that addresses these challenges by combining iterative immuno-fluorescent staining with high dynamic range imaging to facilitate quantitative phenotyping with single-cell resolution.

In this research poster, Canopy Biosciences demonstrates how standard flow cytometry standard (FCS) files are generated from multichannel OME-TIFF images, enabling the identification of cellular phenotypes via flow cytometry-like hierarchical gating. Quantification of results reveals precise expression levels for each marker in the assay in each individual cell in the sample, while maintaining spatial information about each cell.

Advancements to include CosMx® Whole Transcriptome Panel; enhanced technology engine to power CellScape™ for spatial proteomics; expansion to 1000-plex protein assay on GeoMx® DSP; and launch of PaintScape™, a revolutionary platform enabling direct visualization of the 3D genome