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Cell-Free In Vitro Protein Expression

A rapid and flexible protein synthesis system that uses cell lysates rather than living cells to produce proteins, ideal for high-throughput expression of toxic or unstable proteins.

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Creative BioMart

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Cell-free protein synthesis bypasses the need for cell culture and transfection. By providing the necessary transcription/translation machinery in a test tube, it allows for rapid production of proteins in hours. This system is particularly useful for incorporating non-natural amino acids, producing toxic proteins, and for high-throughput screening.

Key Features & Benefits:

  • Very Fast - Protein in Hours, Not Days or Weeks
  • Bypasses Cellular Toxicity and Folding Limitations
  • Ideal for Incorporation of Modified Amino Acids and Isotopes
  • Scalable from Microplates to Milliliter Reactions

Relevant Applications:

  • High-Throughput Protein Expression Screening
  • Production of Toxic or Proteolytically Unstable Proteins
  • Protein Labeling with Stable Isotopes (NMR) or Selenomethionine (X-ray)
  • Synthetic Biology and Protocell Research

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