New Transcreener CD73 Assay for the discovery of therapeutics to enhance tumor immunity
27 Apr 2020
Researchers at BellBrook Labs have developed a high-throughput screening (HTS) assay to discover small molecule modulators for the immunosuppressive ectonucleotidase, CD73. The Transcreener ADO CD73 Assay detects adenosine (ADO), the product of a CD73 reaction, allowing researchers to monitor CD73 activity with simple, mix-and-read fluorescent detection.
Also known as ecto-5’ nucleotidase or 5’-nucleotidase, CD73 is an anchored cell surface protein that plays a critical role in purinergic signaling by catalyzing the hydrolysis of AMP into adenosine and phosphate. Multiple lines of research have shown that adenosine is immunosuppressive in the tumor microenvironment, and ectonucleotidase enzymes have emerged as promising immuno-oncology targets.
The Transcreener CD73 Assay is a competitive immunoassay that uses a coupling enzyme to convert AMP to ADP, which is then detected by the proven Transcreener ADP² Assay. The assay is designed for HTS in miniaturized 384 or 1536 well formats. It provides a quantitative measurement of CD73 enzyme velocity and thus has excellent utility for all aspects of screening and hit-to-lead, including dose-response measurements and mechanistic studies, as well as basic research on CD73 enzymology and regulation.
Targeting the CD73 pathway, in combination with other potentially complementary immune pathways, is a potential strategy to help activate an anti-tumor immune response. The availability of HTS-compatible Transcreener assay methods from BellBrook Labs will accelerate the discovery of inhibitors for enzymes such as CD73, which play a role in tumor immunity and other diseases impacted by adenosine signaling.
Want more of the latest science news straight you your inbox? Become a SelectScience member for free today>>
Related Products
Request Quote for All Products
Transcreener® UDP² Glycosyltransferase Assays
BellBrook LabsThe Transcreener® UDP² Glycosyltransferase Assay Kit directly detects UDP—the conserved product of glycosyltransferase reactions—in a homogeneous mix-and-read format. Compatible with FP, FI, and TR‑FRET readouts, it supports UDP concentrations from 1–250 µM and delivers exceptional HTS performance (Z’ ≥ 0.7) across 96-, 384-, and 1536‑well formats.
Transcreener® AMP²/GMP² Phosphodiesterase Assays
BellBrook LabsThe Transcreener® AMP²/GMP² Phosphodiesterase Assay Kit directly detects AMP or GMP produced by PDEs, ligases, synthetases, ENPP1, and related enzymes using a mix‑and‑read FP or TR‑FRET format—no coupling steps needed. Compatible with 1–1000 µM native substrates, it delivers excellent HTS performance (Z’ ≥ 0.7, ≥100 mP shift) in a one-step, far‑red fluorescence assay.
Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.