MP Biomedicals Accelerates Zika Virus Research by Providing Researchers with Quantitative Sample Preparation, Extraction and Isolation Solutions
26 Jul 2016
Image copyright: mycteria/Shutterstock
Recent Zika virus cases have emphasized the importance of research characterize arboviruses and specifically related members of the genus Flavivirus that are responsible for related diseases including yellow fever, dengue, West Nile virus, and various encephalitises and hemorrhagic fevers.
The FastPrep Suite of Sample Preparation instruments, Lysing Matrix Tubes and Purification Kits provides researchers with quantitative sample preparation, extraction and isolation of nucleic acids, proteins and small molecules from virtually any sample type including insects, infected tissue, soil, water, bodily fluids and feces.
The FastPrep-24 5G is an innovative benchtop bead-beating sample-preparation instrument producing thorough and rapid grinding, lysis and homogenization of routine and resistant samples. The mechanical sample lysis prepares the sample for the isolation of intact DNA, stable RNA and active proteins from all samples related to various arbovirus’ life-cycles.
Examples of downstream viral end-points achievable after FastPrep processing:
- Detection of viral RNA in virtually any sample by using reverse transcription PCR (RT-PCR) to determine infection.
- DNA sequence analysis to characterize symbiont to vector/host relationships for transmission fitness and for vector control strategies
- Nucleic acids and protein analysis to identify/confirm vector taxonomy and phylogeny, and to determine likelihood estimates of vector population infections (MLEs)
The FastDNA SPIN Kit for Soil rapidly and reproducibly isolates pure DNA from all organisms present in soil, fecal or other environmental samples.
FastDNA SPIN Kit for Soil benefits:
- Process up to twice the amount of soil compared to competing kits
- Flexible purification method allows up to 50 ug* or more gDNA recovery from a single sample
- Resulting pure gDNA is inhibitor-free and ready to use in PCR, qPCR, Sanger, NGS and WGS
- FastPrep instruments combined with FastPrep Lysing Tubes deliver quantitative sample lysis that is more efficient than other methods resulting in pure DNA of high MW and superior yield
The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples including water, sediment, sludge and feces. Up to 500 mg soil are processed by FastPrep® with optimized Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as Gram + bacteria; eubacterial spores, endospores and parasitic oocytes; yeast and molds; algae, archea, invertebrates, and decaying plant tissue. The released DNA is purified by the patented GENECLEAN silica-based spin filter method which is scalable in binding capacity, allowing for maximum yields of inhibitor-free gDNA suitable for all downstream applications.
*Difficult samples successfully processed include Gram + bacteria including Bacillus spp., Cornybacterium bovis, Clostridium difficles, Enterococcus faecalis, Staphylococcus spp., Mycobacterium spp., and many Actinomycetes. Fungal DNA from genera such as Aspergillus, Cladosporium, Fusarium, Cryptococcus and Candida have been isolated and this kit will also process diverse samples such as bronchoalveolar lavage and oral washes. Parasitic ova such as Echinococcus granulosa has also been successfully processed making the FastDNA SPIN Kit for Soil the researcher’s choice for metagenomics studies of ecological microbial biodiversity, total microbial biomass quantitation and pathogenic species identification.
Isolation Workflow:
Downstream applications: RT-PCR, nested PCR, Sequencing, Gene Expression, Microarray analysis, Maldi-TOF MS.
Related Products
Request Quote for All Products
FastDNA SPIN Kit for Soil™
MP BiomedicalsIsolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.
FastPrep-24™ 5G
MP BiomedicalsThe FastPrep-24™ 5G Instrument is quite simply the most advanced sample preparation system ever! The only high-speed benchtop homogenizer featuring microprocessor-controlled, Hi-Def Touch Screen Programming makes the 5G the ultimate in ease, speed and performance for sample preparation applications where it is necessary to grind, lyse, or homogenize difficult and routine samples, allowing easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Applications include but are not limited to: biological tissues, fluids and excretions cell cultures environmental sample other inorganic solid matrices. The FastPrep-24™ 5G Instrument uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Samples and buffers are added to Lysing Matrix Tubes containing specialized Lysing Matrix beads, supplied ready-to-use, certified nuclease-free and in a variety of sample type specific compositions. The sample tubes remain securely sealed during the processing and the single use design eliminates cross-contamination. Program parameters are easily set using the touch screen user interface, or choose from many Recommended Programs stored on the 5G's onboard computer. With a simple touch of the "run" button, in less than a minute your samples are completely lysed, super fast, every time! The highest power settings available ensure that DNA, RNA and proteins, even from difficult samples, are quickly liberated into the protective buffer, thus preserving their biological function. FastPrep-24™ 5G Exclusive Features Powerful - Highest speed range available, ideal for a wide range of applications and protects your sample Optimized - Cycle and pause programmable for true walk-away operation Convenient - >70 Recommended Programs for easy optimizations Intuitive - Interactive user-friendly interface and touchscreen Flexible - Optional sample holders allow processing from 2 - 50 mL tubes, under ambient or cryogenic conditions Smart - Integrated USB Port allows for software updates to be installed, and run history data to be downloaded to a laptop Safe - Electromechanical dome latch automatically secures processing chamber, with a simple finger touch Complete - Lysing Matrix Tubes and Purification Kits from One Source
Lysing Matrices
MP BiomedicalsLysing Matrices tubes are plastic tubes containing specific beads designed for homogenization of any sample. The sample material is disrupted through multidirectional simultaneous impacts with the specialized Lysing matrix beads in a bead beater instrument, usually in presence of a lysis buffer. Although Lysing Matrix have been designed to be used with the FastPrep ® system, the standard size of the tubes allows their use with many other bead beaters. The lysing matrix tubes are available in many sizes, ranging from 2ml to 50ml and 96-well plates. The wide range of Lysing Matrix types ensure optimal homogenization of any sample.