HPLC 2009: Phenomenex Column Technologies for Speed and Selectivity

18 Jun 2009

Visit booth #55-57 to see new products for increasing HPLC speed and selectivity for a wide range of applications, including chiral separations and proteomics. In the technical program, look for Phenomenex presentations on separating and purifying chiral compounds.

Simplification of chiral separations has never been easier with Lux columns. Using a unique chlorinated amylose phase, Lux Amylose-2 brings broad selectivity, significantly increasing the successful separations of enantiomers. Lux Amylose-2 compliments the widely popular Lux Cellulose-2, which uses a novel chlorinated cellulose phase, and Lux Cellulose-1, which uses a powerful dimethyl cellulose phase. Together, the successful separation of various chiral compounds can easily be performed, from analytical scale to process purification.

Also new from Phenomenex are six new offerings in its Onyx™ monolithic HPLC column line, including a 150 X 0.05 mm ID choice for proteomics research. This column is optimized for the low flow-rate systems commonly used for LC/MS proteomics analysis and delivers high sensitivity for biomarker discovery. Other new Onyx column sizes include a 0.2 mm C18 column for higher flow rates, a 150 X .2 mm trapping column, a 150 x 0.1 C8 column and two ultra-high-resolution C18 columns at 100 and 200 mm IDs.

Additionally, Phenomenex will present widely used LC solutions:
- Gemini-NX columns for robust reversed phase separations
- Jupiter columns for protein and peptide separations
- Axia hardware for lab-scale purification with improved efficiencies and longer column lifetime


Poster Presentations
• The Changing Landscape of Chiral Separations and Purifications
Tuesday, 30 June
13:00 - 15:30

Onyx Monolithic LC Columns

Phenomenex Inc

Acheive Faster HPLCOnyx monolithic silica-based HPLC columns are designed for high-throughput analysis and the direct injection of dirty or viscous samples. Reduce run times with extremely high flow rates (up to 9 mL/min) "Dilute-and-Shoot” dirty biological samples Scalable from capillary to semi-prep column dimensions Onyx columns deliver high efficiency separations and can be coupled together in series to produce the extreme plate counts needed to separate very complex mixtures.Dilute & Shoot Complex mixtures coupled with dirty matrices, such as serum samples encountered in the pharmaceutical/biotechnology industry, are quite difficult and time-consuming to analyze. With Onyx however, you have the option to dilute-and-shoot your sample directly onto the column because it won't clog due to the macroporous system or cause high backpressures. Flow Rate Flexibility Another unique feature is the ability to use flow rate as parameter for optimizing separations. Onyx columns are responsive to changes in flow rate and can be changed mid run to reduce total run time when certain compounds elute much later. This type of flow rate flexibility gives many options for optimization.

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Jupiter LC Columns for Proteins & Peptides

Phenomenex Inc

RP-HPLC for Protein/Peptide Analysis and PurificationJupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification. A wide variety of phases (C18, C12, C5, C4) and dimensions (capillary-preparative)/bulk media are available to support the majority of separation requirements.Jupiter 300 - 300 Å columns designed to purify intact proteinsJuptier Proteo - 90 Å columns engineered for increased peak capacity and resolution of peptide maps as well as peptide separationsExplore Aeris Core-Shell HPLC/UHPLC columns for ultra-high performance protein/peptide analysis and characterization.

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HPLC 2009: Phenomenex Column Technologies for Speed and Selectivity