New proteoElute nanoLC System and PepSep Advanced nLC Columns showcased at ASMS 2025

At ASMS 2025 Bruker Corporation showcased the new proteoElute™ nanoLC system, featuring ceramic valves, proteoTrap™ trapping columns, and next-generation PepSep^® Advanced nLC columns, further enhancing robustness, sensitivity and proteome coverage in ultra-sensitive proteomics workflows.

The proteoElute™ is ideal for single-cell proteomics, immunopeptidomics, microbiome metaproteomics profiling, and many other proteomics methods, ensuring reliable performance even with limited or very complex biological samples. It features innovative TwinScape™ technology, which continuously monitors system health in real-time and runs background diagnostics. This proactive approach alerts users to potential issues, providing peace of mind when working with precious samples.

Engineered for relentless reliability, the proteoElute™ includes biocompatible ceramic valves, new porous metal filters, and a proprietary trap column that together enhance robustness, chromatographic performance, and permit sample recovery. The new injector and needle wash system reduces carry-over by up to 3x, a critical advantage for confidently avoiding background signals when analyzing ultra-low input samples.

W. Hayes McDonald, Associate Director in the Department of Biochemistry at Vanderbilt University, commented, "The combination of the new proteoElute UHPLC platform along with updated PepSep Advanced columns and the proteoTrap column allows us the flexibility to perform any state-of-the-art proteomics workflow with highest performance. Its remarkable robustness gives us the confidence to execute all proteomics workflows at scale."

For highest sensitivity and performance of the proteoElute™ nLC system, Bruker has introduced a new generation of PepSep^® Advanced nLC columns, engineered for precision, robustness, and speed. The columns deliver sharper peaks and higher-resolution separations for even deeper proteome coverage and consistent performance with challenging biological samples.

In benchmark studies, the PepSep^® Advanced columns demonstrated approximately 20% improvements in peptide IDs and typically 10% improvements in protein IDs.

Combined with CaptiveSpray Ionization (CSI) emitters, users can extend column lifetime by simply replacing the emitter—ensuring long-term performance and cost efficiency. PepSep^® columns provide the reliability and depth needed for analyzing complex tissue digests or low-input samples.

Lynn Spruce, the Technical Director at the Children’s Hospital of Philadelphia (CHOP-Penn) Proteomic Core Facility, said, "The new PepSep Advanced columns have proven to be robust, and they consistently deliver reproducible chromatography. They produce sharper, more symmetrical peaks with no observable tailing, resulting typically in 7–8% increases in protein group identifications. Combined with the convenience of interchangeable emitters and reasonable pricing, it has become our go-to nLC column. It’s essentially plug-and-play."

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