Fiber-optic surface plasmon resonance is a technique that was developed in the MeBioS (Mechatronics, Biostatistics, and Sensors) division at the University of Leuven in Belgium. It uses fiber-optic probes to upgrade the well-established surface plasmon resonance technique for bioanalysis, allowing sensors to be dipped into the sample, greatly improving the ease of use.
FO-SPR has been honed by FOx Biosystems, a spin-off company from MeBioS, and integrated into their flagship instrument, the White FOx. This enables users to generate high-quality, cost-effective biomolecular data, fast, and over a wide range of applications, even in the most challenging samples.
Below are some of the benefits of this approach.
Surface plasmon resonance (SPR)
SPR delivers fast, precise, and accurate information about biomolecular interactions without the need for labels. It can provide data on target concentration, affinity, and kinetic interactions, and is commonly used for a wide range of purposes, including protein and antibody characterization, biologics development, therapeutic drug monitoring, allergen testing in food, etc.
However, the majority of microfluidics-based SPR platforms are cumbersome to use, requiring time-consuming sample preparation or large quantities of precious sample. FO-SPR overcomes these obstacles by allowing the sensors to be simply dipped into the sample, providing a user-friendly, versatile system with minimal sample requirement.
Fiber-optic SPR probes can simply be dipped into the sample of interest. This allows users to benefit from the speed and precision of SPR analysis while providing additional advantages such as ease of use and the wide range of potential analyses that can be carried out.
Fast SPR signal amplification
Using gold nanoparticles coated with antibodies, sandwich assays can be carried out without the lengthy enzymatic incubations required by ELISA. This instantly amplifies the SPR signal, while providing the same sensitivity as ELISA. While microfluidic systems suffer from cross-contamination when using strong signal enhancement reagents like gold nanoparticles, the fiber optic dip-in read assay overcomes these issues, thereby avoiding the need for extensive cleaning procedures.
The standard surface chemistry and interchangeable nature of the probes mean that FO-SPR is a highly versatile technique, capable of analyzing a wide range of targets on one instrument.
This versatility of use places high demands on the software, making software an important part of the FO-SPR package. The open data format offers users the ability to perform data analysis with standard data-handling software, which provides the freedom to analyze data in a personalized manner. Furthermore, an intuitive, visual interface makes it simple to set up, adapt and save protocols, depending on the assay requirements.
Main features of FO-SPR:
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