Animated Protocols for Phospho-Protein Analysis

R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor the presence of cytokines and cytokine receptors as well as Fluorokine® kits; labeled cytokines that can be used to monitor the presence of cytokine receptors. Features of the Flow Cytometry Kit:• Simultaneous detection multiple different targets • Rapid & efficient • Single step staining R&D Fluorokine Receptor Detection Kits employ recombinant biotinylated ligands to quantitatively determine cytokine receptor density using flow cytometry. These simple kits allow the user to estimate the percentage of cells within a population which express the receptor of interest. In addition, cell surface receptor density can be assessed. Flow Cytometry Kit Contents (Multi-Color):• Fluorochrome conjugated antibodies • Isotype controls • All necessary buffers Flow Cytometry Kit Contents (Fluorokine Receptor): • Biotinylated recombinant, species specific ligand • Avidin conjugated with fluorescein (at an f:p ratio of 5:1) • Negative control reagents • Blocking antibody • Wash buffer concentrate

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays include:R&D Systems Human Phospho-RTK Antibody Array (Catalog # ARY001)Detect the relative site-specific phosphorylation levels of 42 different receptor tyrosine kinases(RTKs). R&D Systems Human Phospho-MAPK Antibody Array (Catalog # ARY002) Detect the relative site-specific phosphorylation levels of 19 different intracellular serine/threonine kinases. R&D Systems Human Phospho-Kinase Antibody Array (Catalog # ARY003) Detect the relative phosphorylation levels of 46 intracellular serine/threonine/tyrosine kinases. R&D Systems Human Phospho-Immunoreceptor Antibody Array (Catalog # ARY004) Detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors. R&D Systems Human Cytokine Antibody Array, Panel A (Catalog # ARY005) Detect the relative levels of 36 cytokines, chemokines, and acute phase proteins simultaneously. R&D Systems Mouse Cytokine Antibody Array, Panel A (Catalog # ARY006) Detect the relative levels of 40 cytokines, chemokines, and more simultaneously. R&D Systems Human Angiogenesis Antibody Array (Catalog # ARY007) Detect the relative levels of 55 angiogenesis-related proteins simultaneously. R&D Systems Rat Cytokine Antibody Array (Catalog # ARY008) Detect the relative levels of 29 cytokines, chemokines and more simultaneously. R&D Systems Human Apoptosis Antibody Array (Catalog # ARY009) Detect the relative levels of 35 apoptosis-related proteins simultaneously. R&D Systems Human Pluripotent Stem Cell Antibody Array (Catalog # ARY010) Detect the relative levels of 15 pluripotent stem cell markers simultaneously.