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Wild type Taq can only do so much but Kapa SYBR® FAST qPCR Pushes the Boundaries of RT-PCR Even Further!

13 Apr 2009
Samantha Rosoman
Campaign Coordinator

Product news

Now available from Anachem, the NEW KAPA Biosystems SYBR® FAST qPCR Kit contains a novel DNA polymerase evolved specifically for real-time PCR.

The reaction efficiency of qPCR is critical for accurate and reliable gene quantification. Wild type Taq DNA polymerase is often inhibited by real-time PCR reaction conditions, leading to significant loss in specificity and sensitivity of the assay. KAPA SYBR® FAST qPCR Master Mix contains a novel DNA polymerase engineered to perform optimally in stringent qPCR reaction conditions. It exhibits dramatic improvements in signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity and sensitivity.

The novel DNA polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC and AT-rich templates. The kits are ideally suited for gene expression analysis, microarray validation, low copy gene detection, gene knockdown validation.

Whichever real-time PCR machine you are using Kapa SYBR Fast qPCR is available in a compatible formulation. With these kits there is no need for lengthy re-optimisation. Just substitute the master mix and run a side by side comparison to see for yourself the superior results. Its that simple!

To request your FREE sample contact us now!!

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Wild type Taq can only do so much but Kapa SYBR® FAST qPCR Pushes the Boundaries of RT-PCR Even Further!