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Trending in 3D cell culture: From targeting colorectal disease to tumor heterogeneity and patient-derived organoids

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Discover the latest technologies and techniques that are revolutionizing 3D cell culture

26 Feb 2020
Carrie Haslam
Associate Editor

Editorial article

With 3D cell cultures being used increasingly for basic research and drug discovery, advancements in the field come thick and fast. From patient-derived colon cancer organoids and multidrug-resistant organisms to 3D imaging technology, keep up to date with some of the latest developments, techniques, resources and technologies in 3D cell culture in this round-up article. Plus, an expert explains the importance of the cellular microenvironment in the development of efficient drugs against complex disease.

Article: Targeting colorectal disease through patient-derived colon cancer organoids

Dr. Asunción Fernández-Barral is using 3D cell culture to uncover the link between vitamin D deficiency and intestinal stem cells

In this exclusive SelectScience interview, we talk with Dr. Asunción Fernández-Barral, Instituto de Investigaciones Biomedicas "Alberto Sols", CIBERONC, Madrid, Spain, to find more about her research into the regulatory role of vitamin D in relation to human colon stem cells and its implications in colorectal cancer. Read now>>

Article: 3D patient-derived cell models reflect tumor heterogeneity of clear cell renal cell carcinoma

Unraveling the causes and consequences of tumor cell heterogeneity is a main research goal for Dr. Hella Bolck, senior research fellow in the Department of Pathology and Molecular Pathology at the University Hospital Zurich. Studying the remarkable molecular and phenotypic variability of tumor cells is a key focus. By considering the heterogeneity of cells within individual tumors, Bolck’s research aims to help develop better strategies to treat cancer. Read now>>

eBook: Live-Cell Analysis: From Culture to Assay

In this application eBook, we look at the interconnected cellular workflows and continuous analysis cycle of cell culture to show how cutting-edge technologies from Sartorius deliver smarter cell monitoring. Download now>>

Application: 3D analysis of co-culture cancer spheroids using NoviSight™ software

In this application note, Olympus uses NoviSight 3D analysis software and the FLUOVIEW® FV3000 confocal laser scanning microscope to classify the types of cells contained in co-culture cancer spheroids and analyze cell-type-specific drug responsiveness. Download method>>

Application: Next-generation 3D patient-derived cell culture services

Reconstructed bone marrow and 3D cell culture services from zPREDICTA enable accurate testing of therapeutic agents against acute myelogenous leukemia. Download method>>

Article: The importance of the cellular microenvironment in the development of more efficient drugs against complex diseases

Dr. Nathalie Maubon launched HCS Pharma in 2014, inspired by an idea: in order to obtain and select more effective drugs, more relevant cellular models are needed for screening. In this exclusive interview, we speak with Maubon to find out more. Read now>>

Webinar: Multidrug-resistant organisms: Improving the time to effective treatment

In this SelectScience webinar, Dr. Jose Alexander, Director - Clinical Microbiologist at AdventHealth Orlando, discusses two key considerations when tackling the pressing issue of multidrug-resistant organisms. Watch now>>

Video: Increasing the accuracy of testing for anti-cancer therapeutics with Tecan's D300e Digital Dispenser

In this new video filmed at SLAS2020, zPREDICTA CEO and founder Julia Kirshner discusses the benefits of using Tecan's D300e Digital Dispenser for combination treatments in cell cultures to develop laboratory models for more accurate testing of anti-cancer therapeutics. Watch now>>

IHC staining on 3D models reveals protein expression of hypoxia markers

Application: Analysis of RNA transcript levels reveals upregulation of hypoxia markers for pancreatic cancer cells cultured in 3D

Pancreatic ductal adenocarcinoma (PDAC) is a notoriously aggressive tumor type due to its high levels of metastasis and recurrence and is the fourth leading cause of cancer-related deaths in the Western world. In this study, the PDAC cell line PANC-1 was seeded at densities ranging from 500 to 5,000 cells in Corning spheroid microplates and the relative expression of hypoxia markers HIF-1α, GLUT-1, and CA IX were analyzed compared to 2D culture using RT-qPCR to assess the correlation between spheroid size and hypoxia marker expression. Download method>>

Article: Cut through the haze: Can the latest 3D imaging technology help you remove out-of-focus blurring?

In this article, discover how Computational Clearing technology from Leica Microsystems quickly resolves the longstanding challenge of blurry contrast when imaging thick sections. Read now>>

Check out our 3D Cell Culture Special Feature page for more>>

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Antibiotic ResistanceAntibiotic resistance poses a significant threat to public health driving innovation and change through antibiotic stewardship efforts. Ongoing research and diagnostic efforts focus on new treatments and detection methods. Explore antibiotic resistance research and diagnostic tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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