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TIGIT Receptor Products for Immunotherapy Research

AMSBIO
7 Jul 2016
Alex Waite
Editorial Assistant

Product news

AMSBIO has launched a range of new TIGIT receptor products including a Jurkat cell line, Homogeneous assay kits and recombinant proteins for immunotherapy research.

Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor expressed on the surface of human T cells and NK cells that binds to CD155 and CD112 present on the surface of dendritic cells. The binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have known to increase the immune response, particularly in the case of certain cancers.

A new recombinant Jurkat cell line is now available from AMSBIO that constitutively expresses a full length human TIGIT, and a firefly luciferase gene under the control of nuclear factor activator T cells (NFAT) response elements. Both the TIGIT and NFAT constructs have been stably integrated into Jurkat cells. The TIGIT-specific NFAT response has been validated using a TIGIT ligand (CD155 PVR) and TIGIT expression confirmed using Western Blot methodology. This cell line is ideal for high throughput screening (HTS) to identify antagonistic monoclonal antibodies targeting either TIGIT or its ligands, such as CD155 in a cellular context.

AMSBIO has also introduced two TIGIT Homogeneous assay kits, a CD112 kit to measure the inhibition of TIGIT binding to CD112 (PVRL2/Nectin-2), and a CD155 kit designed to measure the inhibition of TIGIT binding to CD155. Both kits come in a convenient AlphaLISA® format with sufficient reagents and buffers to perform a total of 384 reactions. Each kit requires just three simple steps on a microtitre plate therefore are consequently quick and easy to use.

To assist in the study of protein binding and for screening small molecules and antibodies, AMSBIO has additionally launched Fc Fusion TIGIT and biotinylated Fc Fusion TIGIT recombinant proteins.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Therapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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