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Thermo Fisher Scientific Offers Improved Immunoprecipitation Kit for Use with Antibodies from Any Species or Subclass

3 May 2009
Veronica Austin
Marketing / Sales

Product news

Thermo Fisher Scientific, the world leader in serving science, today announced the availability of the Thermo Scientific Pierce Direct Immunoprecipitation (IP) Kit. The kit replaces the use of immobilized Protein A or Protein G with a method for direct covalent attachment of antibodies to the beaded agarose resin, representing a significant advance in IP technology that allows the use any species or subclass of purified antibody.

The Pierce® Direct IP Kit procedure requires only 2-10 micrograms of purified antibody, but is easily scalable. The short coupling method (1-2 hours) covalently attaches antibody to the resin. This irreversible attachment results in minimal contamination of the purified protein and allows the resin to be reused several times. The purified protein obtained using the kit is compatible with mass spectrometry and other downstream applications such as enzymatic assays.

The Pierce Direct IP Kit includes convenient spin columns that eliminate resin loss and provide for efficient separation of solutions. The kit also supplies cell lysis buffer and sufficient reagents to perform at least 50 antibody immobilizations and IP experiments using 10 microliters of immobilized antibody support.

The Pierce Direct IP Kit, along with the Pierce Classic IP and Pierce Crosslink IP Kits, is a new family of products featuring improved immunoprecipitation methods.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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