Thermo Fisher Scientific Offers Improved Classical Immunoprecipitation Kit

Thermo Fisher Scientific, the world leader in serving science, announced the availability of the Thermo Scientific Pierce Classic Immunoprecipitation (IP) Kit. The new kit provides an optimized protocol and buffers to perform highly efficient reactions that require a minimal amount of antibody (2-10 micrograms). The four-step IP reaction is complete in less than one hour.

The Pierce® Classic IP Kit uses a high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of antibodies. The IgG Elution Buffer supplied provides milder and less denaturing recovery of antibody-antigen complexes, facilitating other downstream applications such as enzymatic analysis and mass spectrometry. The microcentrifuge spin column format ensures effective washing and separation of samples from the beaded agarose affinity resin.

The Pierce Classic IP Kit includes lysis/bind/wash buffer and elution buffers, control agarose resin, sample loading buffer and spin columns. The kit supplies sufficient reagents for 50 IP reactions using 10 microliters of immobilized antibody support.

The Pierce Classic IP Kit, along with the Pierce Direct IP and Pierce Crosslink IP Kits, is part of a new family of products featuring improved immunoprecipitation methods. For more information on these kits, visit the article webpage.