Thermo Fisher Scientific offers great savings in ‘Winter Warmer’ promotion

Thermo Scientific Red Hot Taq DNA Polymerase consists of ThermoPrime Taq DNA Polymerase with an inert red dye to facilitate accurate low volume pipetting and as an indicator of enzyme addition. This dye has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Highlights • Red colouring facilitates low volume pipetting • Reaction mix becomes pink on enzyme addition for easy identification Applications • Amplifying fragments up to 5 kb • Routine PCR Includes • Red Hot Taq DNA Polymerase • 10X PCR Buffer • 25 mM MgCl2

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands. ■ Hot-Start modification ensures stringent specificity ■ Safe for room temperature reaction setup ■ Enhanced enzyme stability for increased yield and sensitivity ■ Advanced buffering system minimizes PCR optimization steps ■ High Performance Buffer formulation for GC-rich and other complex templates Thermo-Start is available in a range of formats and pack sizes to suit any workflow. Format Options: ■ Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffer, and 25mM MgCl2 solution, each in separate vials. dNTPs must be purchased separately. ■ Complete PCR Kits include all the components needed to perform PCR, including an HPLC-purified dNTP mix. Complete PCR kits are a convenient option that still allows for MgCl2 optimization. ■ Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format as they significantly reduce assay set-up steps and minimize the risk of sample contamination. ■ Premixed MgCl2 Kits have MgCl2 premixed into the PCR buffer and will be at 1.5mM concentration in the final reaction. ■ High Performance Buffer Kits are specially formulated for GC-rich or other complex targets with a high degree of secondary structure. Storage Conditions: Store at -20°C for long-term storage. Stable at +4°C for up to four weeks. Avoid freeze-thaw cycles. Compatible with: Thermo-Start Taq Reddymix Kits and Master Mixes