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Thermo Fisher Scientific Introduces Two New Western Blotting Kits for Fast Detection at Femtogram Levels

15 Feb 2010
Sarah Sarah
Marketing / Sales

Product news

Thermo Fisher Scientific today introduced the latest additions to its extensive line of fast Western blot detection products, the Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal® West Dura Substrate and the Pierce® Fast Western Blot Kit, SuperSignal West Femto Substrate.

These new kits provide optimized reagents and protocols that decrease the time for Western blot detection from four hours to approximately 60 minutes, without loss in sensitivity. The kits require minimal hands-on time and require no specialized equipment.

The kits include popular varieties of SuperSignal Chemiluminescent Substrate, a highly sensitive enhanced substrate for detecting horseradish peroxidase (HRP). The SuperSignal West Dura Substrate’s intense signal output enables detection of femtogram amounts of antigen. The SuperSignal West Femto Substrate offers the highest level of sensitivity in the product line, achieving detection in the low to mid femtogram range of antigen.

Both Pierce Fast Western Blot Kits can be used with either nitrocellulose or PVDF membranes. The sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed. Separate kits for use with either a mouse or rabbit primary antibody are available.

The kits are available in a trial size sufficient to perform two Western blots (8 x 10 cm). A larger kit with reagents for 10 Western blots is also available.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Introduces Two New Western Blotting Kits for Fast Detection at Femtogram Levels