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Thermo Fisher Scientific Announces New Western Blotting Accessories

12 Oct 2010
Sarah Sarah
Marketing / Sales

Product news

Thermo Fisher Scientific introduceds Thermo Scientific SuperSignal Western Blot Enhancer and two varieties of Thermo Scientific SuperSignal Molecular Weight Protein Standards.

SuperSignal® Western Blot Enhancer increases signal intensity and sensitivity three- to ten-fold compared to conventional Western blotting methods.The solution significantly reduces background noise, allowing detection of antigens that are weakly immunoreactive or of low abundance. These features also minimize the number of overexposed x-ray films produced, saving researchers time.

SuperSignal Western Blot Enhancer can be used with both PVDF and nitrocellulose membranes and is compatible with fluorescent, chemiluminescent and chomogenic detection methods. The kit contains sufficient reagents for 25 mini-blots. A kit for two mini-blots is also available.

The ready-to-use SuperSignal Molecular Weight Protein Standards contain a mixture of eight recombinant proteins with molecular weights ranging from 150K to 20K. Each protein contains a IgG-binding site and the amounts are adjusted to yield comparable band intensities after gel electrophoresis and blotting. The standards contain a pink tracking dye to easily monitor electrophoresis. The SuperSignal Molecular Weight Standards are compatible with chemiluminescent, fluorescent and chromogenic Western blot detection methods.

The SuperSignal Enhanced Molecular Weight Protein Standards have the same properties as described above but are formulated specifically for use with mouse antibodies.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Announces New Western Blotting Accessories