Tecan and amaxa combine their technologies and create automated systems for high throughput transfection of primary cells

Tecan and amaxa have successfully integrated the amaxa Nucleofector^® 96-well Shuttle^® System with the Tecan Freedom EVO^® liquid handling workstation, allowing fully automated, high throughput transfection of primary cells and difficult-to-transfect cell lines, including non-dividing neurons and T cells, with siRNA, DNA and other substrates. The development is ideal for large-scale studies that involve high throughput transfection stages, such as RNAi-based screening for drug development.

The freely configurable Freedom EVO workstation provides precise and reliable liquid handling and can accommodate a wide range of modules, including a robotic manipulator arm to transfer labware to and from devices such as the 96-well Shuttle. The 96-well Shuttle relies on unique electroporation technology for transfection of primary cells with high efficiency and excellent survival rates. Integrating the 96-well Shuttle with the Freedom EVO workstation entirely removes all manual steps for cell preparation and transfection, allowing 96-well microplates to be completely processed in just minutes, depending on the protocol used. The integration enables, for the first time, transfection of primary cells in a fully automated environment, providing a valuable tool for research and drug discovery studies.

Please note: Not for use in clinical diagnostics.