Sigma's new Prestige Antibodies™ are the Most Highly Characterized Antibodies in the Industry

3 Apr 2008

Product news

Life scientists require well-characterized antibodies as essential tools for protein studies, global proteomics analysis, as well as for clinical diagnostics. Although a large number of antibodies are commercially available through many vendors, specific antibodies still do not exist for the majority of human proteins and there is a lack of universal protocols for antibody validation.

The Swedish Human Proteome Resource (HPR) project, using an antibody-based proteomics approach, is exploring the entire human proteome through a high-throughput antibody production process. The HPA program generates protein expression profiles from the majority of human tissues and presents the data as immunohistological images, annotated and made publicly available via an open access database, the Human Protein Atlas (www.proteinatlas.org). The Human Protein Atlas displays a collection of over 2.9 million IHC images of normal and cancer tissues, of cell lines and primary cells.

Sigma® Life Science has entered an exclusive collaboration with the HPR and Atlas Antibodies AB to offer these antibodies through our Prestige Antibodies™ portfolio. Each antibody is highly validated in multiple assurance steps to ensure that they are the most specific products on the market. The antibodies are designed to have low cross-reactivity to other human proteins and to work in a variety of applications and tissue preparations. Prestige Antibodies are optimized on a single protocol and tailored for high throughput operations, providing time and cost savings to the researcher.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.
Sigma's new Prestige Antibodies™ are the Most Highly Characterized Antibodies in the Industry