Shimadzu Introduces Sensitive Fluorescence Detectors for HPLC Systems

19 Apr 2010
Sarah Sarah
Marketing / Sales

Product news

Shimadzu’s new RF-20A and RF-20Axs fluorescence detectors provide the ultimate in sensitivity and validation support functions in a wide range of applications from conventional to ultra-fast LC analysis. Utilizing a newly designed optical system, the RF-20A and RF-20Axs have achieved the highest sensitivity levels of any HPLC fluorescent detector.

A water Raman S/N ratio of at least 1,200 for the RF-20A and 2,000 for the RF-20Axs makes these detectors powerful tools for tests demanding analysis of trace-level components while retaining the acquisition speeds necessary for ultra-fast analysis. Because fast response is necessary to follow the sharp peaks in ultra-fast analysis, the RF-20A and RF-20Axs provide 10 ms response. The quick response time permits ultra-fast LC without loss of separation.

Additionally, simultaneous testing of multiple components requires detection at optimal wavelengths. These detectors allow ultra-fast, highly sensitive multi-component analysis using wavelength switching via a time program.

The RF-20Axs also features a temperature-controlled cell with a cooling function. This maintains a constant detector cell temperature, even if the testing environment’s room temperature fluctuates, ensuring reproducibility without any drop in sensitivity.

For easy maintenance, each detector’s xenon lamp and flow cell can be replaced at the front panel. The standard flow cell or semimicro flow cell can be replaced quickly without any tools. Additionally, the xenon lamp life has been extended to 2,000 hours, four times longer than previous Shimadzu lamps. This reduces running costs and downtime due to maintenance.

Specialized application systems for amino acid, reducing sugar and carbamate pesticide analysis are also available with either the RF-20A or RF-20Axs detector.

Links

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Raman SpectroscopyRaman spectroscopy is used to discern the vibrational and rotational states of molecules and hence the chemical composition of a sample by measuring the inelastic scattering of monochromatic light. Explore a range of Raman spectrometers, including handheld/portable Raman spectrometers for QC/QA labs and in situ spectrometers for processes. Conduct Raman imaging for microanalysis of mixed samples using a Raman microscope. Raman spectrographs are also available. Find the best Raman spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Shimadzu Introduces Sensitive Fluorescence Detectors for HPLC Systems