Screening and Characterization of Rabbit Polyclonal Antibodies on a Single Chip

10 Dec 2007

The primary goal of antibody production in laboratory animals is to obtain high titer, high affinity antisera for use in experimentation or diagnostic tests. However, detection and monitoring of the purification and quality of these polyclonal antibodies can be both tedious and troublesome.

Using the fully automatic Attana A100® C-Fast and Attana´s new Rabbit Antibody Capturing Surface allows for a straight-forward and quick solution to find the best antibodies from your antisera without the need of purification (see downloadable file).

The use of the Attana A100® provides detailed interaction kinetics and selection of antibodies, without the need of labels, resulting in faster and more cost effective research.

A100 C-Fast

Attana AB

The Attana A100® C-Fast system provides kinetics, off-rate screening and selection of antibodies, without the need of labels, resulting in faster and more cost effective research. Key System Features and Benefits: A100 – Label free on-line kinetics plus much more The A100 system is optimized for label-free, cost efficient, on-line kinetic studies (on/off rates and affinity), which provides considerable advantages over end-point assays such as ELISA. In addition, you can analyze crude samples without purification, making it easier to characterize and select monoclonal antibodies directly from cell culture media. A100 system also features integrated and accurate temperature control. This both enables thermodynamic studies and optimizes the environment when working with temperature sensitive reagents. A100 – C-Fast autosampler maximizes productivity The enhanced features of the A100 C-Fast system maximize productivity by allowing unattended analysis of up to 192 samples. The A100 C-Fast system can screen 200 combinations of antibody sandwich pairs in less than a day. Fast and intuitive set-up includes pre-programmed methods and templates further demonstrating the system’s efficiency. A100 – Proven, reliable and versatile technology The A100 is based on the proven, reliable and versatile QCM technology. Featuring user-friendly Attester® software for instrument control, data collection, data selection, reference subtraction, and data preparation for kinetic analyses, A100 delivers high quality data. A100 – Cost effective research The A100 is a cost-effective alternative for accessing in-depth analyses of molecular interactions, delivering high quality data for a variety of applications.

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