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SafeView - Safer Alternative to Ethidium Bromide

NBS Biologicals Ltd.
14 May 2008
Lizz Mayers
Marketing / Sales

Product news

SafeView is a new, safe nucleic acid stain for the detection of double-stranded DNA, single-stranded DNA and RNA in Agarose gels. This dye replaces Ethidium Bromide (toxic, potential mutagen) commonly used in Agarose gel electrophoresis.

SafeView is as sensitive as Ethidium Bromide and is used in the same way for electrophoresis. SafeView emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This stain has two fluorescence excitation maxima when bound to nucleic acid, at approx. 290nm and 490nm.

SafeView is non-carcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chomophilous erythrocyte micronucleus test and mouse spemary spermatocyte chromosomal aberration test. SafeView can be disposed of as any other non-carcinogenic fluorescent dye (eg Acridine Orange).

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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SafeView - Safer Alternative to Ethidium Bromide