Roche Launches Complete His-Tag Purification Column for Convenient One-step Protein Purification
25 Jun 2013
Roche today announced the launch of its new cOmplete His-Tag Purification Column for researchers performing histidine-tagged protein purification from lysates. The new column uses Roche’s proprietary nickel-chelate chemistry and is compatible with commonly used reducing agents (DTT), metalloproteinase inhibiting reagents such as EDTA, and different buffer and salt environments. This allows researchers to choose optimal buffer conditions for the target protein in a convenient pre-packed format.
“We have received very positive feedback from pilot users who appreciate the flexibility of being able to purify proteins under protein protecting conditions,” said Ruedi Stoffel, Head of Biochemical Reagents & Custom Biotech. “It enables our customers to apply any buffer required to obtain proteins of high purity, stability and function.”
Following the purification step, the new column does not require buffer exchange or resin recharging therefore avoiding a costly and time consuming process. In addition, the resin’s minimized nickel ion leakage not only reduces toxic nickel waste, but also stabilizes the target protein by preventing nickel ions from catalyzing protein oxidation.
Thanks to the additional format, researchers can choose their preferred method of purification. The cOmplete His-Tag Purification Resin can be used for batch purification as well as on automated systems based on FPLC. Pre-packed cOmplete His-Tag Purification Columns are available in two sizes (1 ml and 5 ml) and are compatible with standard instruments for protein purification.
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cOmplete His-Tag Purification Column - Prepacked columns with EDTA-compatible resin for the purification of poly-histidine-tagged proteins. cOmplete His-Tag Purification Columns are available in 2 different sizes and prepacked with cOmplete His-Tag Purification Resin. cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purifications of His-tagged proteins from total lysates. cOmplete His-Tag Purification Column Features: Use the buffer conditions best suited to your protein - Keep your protein comfortable and let it, not your purification resin, determine whether you use DTT, EDTA, or other buffer substances. Repeatedly obtain highly pure protein - Single step purification without resin recharging. Protect your protein from toxic nickel - Reduce protein oxidation and aggregation caused by resins that leach nickel. Work in a safe and eco-friendly environment - Avoid handling of toxic nickel and completely eliminate disposal costs.
cOmplete His-Tag Purification Resin
cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The wide choice of compatible ingredients makes it easy to optimize buffers for high protein stability and solubility and to protect your proteins from proteases and oxidation. Stabilize your protein with DTT and EDTA. Use any buffer with EDTA or DTT for the highest protein stability without loss of capacity. Produce large amounts of highly purified proteins. High binding capacity and specificity are ideal for one-step purifications. Generate less toxic waste. Negligible nickel leakage reduces toxic waste and avoids nickel recharging.