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Robust detection, quantification & purification of your protein tags

PROGEN
27 Oct 2020
Edward Carter
Publishing / Media

Product news

Get ready for robust detection, quantification, and purification of your protein tags with PROGEN’s wide range of anti-tag antibodies against the most commonly used tags (His, DDDDK, GST, HA, myc, GFP & Strep). The high-affinity antibodies facilitate reliable detection and purification of tagged proteins for protein-protein interaction studies, posttranslational modification studies, and many more.

The use of recombinant proteins for cell biological studies including e.g. protein-protein interactions, posttranslational modifications, expression dynamics or localization has increased in recent years. To allow the analysis at the molecular level suitable tools including antibodies specifically targeting the protein of interest are needed but not always available. Therefore, a convenient molecular method is the fusion of known protein tags with high affinity to a specific ligand, to a recombinantly expressed protein.

PROGEN´s tag antibodies are available for the most commonly used protein tags, e.g. His-tag, GST-tag and many more. All of the tag antibodies bind with high affinity to the respective protein tag allowing robust and highly specific detection and purification. PROGEN´s tag antibodies are suitable for several applications including western blot (WB), immunofluorescence (IF), immunocytochemistry (ICC), immunoprecipitation (IP) and ELISA.

All of the tag antibodies are available in different quantities including either 25µg or 100µg to allow you to choose what you really need for your experimental setup.

Key features:

  • Robust & highly specific detection, quantification & purification
  • Available for the most commonly used protein tags (e.g. His-tag)
  • High affinity to the respective protein tag
  • Suitable for many applications (e.g.WB, IF, IP, and ELISA)
  • Available in different quantities (25µg & 100µg)

Relevant applications:

  • Protein-protein interaction studies
  • Posttranslational modification analyses
  • Expression dynamic studies
  • Localization studies
  • Detection & purification
  • Western blot (WB)
  • Immunocytochemistry (ICC)/immunofluorescence (IF)
  • Immunoprecipitation (IP)

Find out more about Progen's antibodies here:

Links

PROGENCompany website

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.Protein Purification

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