Reproducible Isolation of High Quality RNA with INTEGRA’s VIAFLO 384 Electronic Pipette
22 May 2013
INTEGRA has released a new video demonstrating a solid phase extraction (SPE) microplate method for reproducible, high throughput isolation of high-quality DNA-free total RNA.
Large scale gene expression screening projects require reliable high-throughput methods to isolate high quality RNA from a large number of samples. To meet this need, a growing number of commercial 96-well format RNA isolation kits are now available. Typically such kits are compatible with commonly used 96-well plate vacuum manifolds enabling the extraction (isolation) procedure to be accelerated.
The video shows how researchers use a VIAFLO 384 handheld electronic pipette in conjunction with vacuum manifold, 96-well solid phase extraction (SPE) microplate and RNA isolation kit to reproducibly extract high quality total RNA from cultured cells and bacteria.
The VIAFLO 384 is a handheld 384-channel electronic pipette that offers all the benefits of increased sample throughput as well as lower sample and reagent use without the expense of having to invest in a robotic liquid handling system. Designed to be used just like a standard handheld pipette the VIAFLO 384 requires no special skills or training to operate it. The VIAFLO 384 features INTEGRA's popular Touch Wheel™ pipette user interface enabling simple and fast programming of a wide range of pipetting modes including repeat dispense, serial dilute and mixing routines.
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VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette
INTEGRA BiosciencesINTEGRA Biosciences’ VIAFLO 384 increases productivity of sample preparation in microplates and supports 24, 96 and 384 channel pipetting heads. VIAFLO 384 is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. With a choice of ten interchangeable pipetting heads, the unit quickly adapts to any 24, 96, 384, or even 1536 well pipetting application in a volume range of 0.5 μl to 1250 μl. VIAFLO 384 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 384 can be used for sample transfers from reservoir to plate, plate to plate or within plates. VIAFLO 384 Features and Benefits: VIAFLO 384 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. Various pipetting modes are available for increased productivity and application customization.