Quality control of NGS libraries with daisy chains

The Agilent automated electrophoresis solutions offer fast and reliable quality control of next-generation sequencing (NGS) libraries. The Bioanalyzer, TapeStation, and Fragment Analyzer systems with dedicated high sensitivity assays allow unambiguous detection of daisy chains and accurate sizing of the target library. In this application note, Agilent Technologies provides a recommendation for which assay to employ to reliably visualize daisy chain products in NGS libraries. Furthermore, it emphasizes the consistency between the instruments and reproducibility of analysis confirmed by results on a set of two-fold serial dilutions.