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Protein Separation Simplified with Powerful Benchtop System

NuSep
10 Dec 2008
Samantha Rosoman
Campaign Coordinator

Product news

NuSep’s MF10 offers complete flexibility for a wide range of laboratory scale protein separations on a versatile and easy to use benchtop instrument. The MF10 can fractionate or concentrate protein on the basis of size and / or charge, as well as differentially deplete samples of charged or uncharged interfering compounds.

A user configurable cassette accepts up to 8 size exclusion membranes (conveniently color coded and ranging from 5KDa – 500KDa) for the separation of one or two samples (50ul – 1ml) into 2-8 fractions. The scientist has complete control of buffer, pH, eletrophoretic conditions and temperature allowing proteins to be separated under both native and denaturing conditions with separated fractions quickly and easily recovered by pipette.

The introduction of the MF10 offers the researcher a versatile and powerful system for protein fractionation, sample preparation for 2-D PAGE, Mass Spec as well as a growing number of applications that include rapid desalting of samples, removal of charged and uncharged species from Urea or detergents and the control of denaturing conditions for proteomic studies. Application notes available on request.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Protein Separation Simplified with Powerful Benchtop System