Promega Introduces MethylEdge Bisulfite Conversion System for DNA Methylation Analysis
12 Feb 2013Promega Corporation announced today that it has launched MethylEdge™ Bisulfite Conversion System. The system offers an efficient method for rapidly performing bisulfite conversion and DNA clean-up within two hours, while maintaining DNA integrity to provide more intact, converted DNA for a variety of sensitive applications.
MethylEdge requires only small amounts of input genomic DNA (100pg-2µg). Using optimized conversion reagents, clean-up and recovery methods, the system converts DNA at =90% efficiency with minimal fragmentation. The resulting DNA is suitable for use in downstream applications such as PCR, array, or sequencing assays.
The kit includes all reagents necessary to perform 50 bisulfite conversion reactions. All MethylEdge reagents can be stored at room temperature and require minimal up-front preparation. Control DNAs are also available separately and can be used as standards in both the conversion reaction and post-conversion analysis.
The Promega MethylEdge Bisulfite Conversion System complements Promega nucleic acid purification kits for a wide variety of sample types and throughput levels, quantitation chemistries for both pre- and post-conversion, and Promega amplification technologies for detection, providing a quality workflow solution to DNA methylation sample preparation.
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MethylEdge™ Bisulfite Conversion System
Promega Corp.The MethylEdge™ System provides an efficient method to rapidly perform bisulfite conversion with minimal DNA fragmentation, giving you more intact, converted DNA for downstream applications. Start with as little as 100pg of input DNA for those precious, limited samples. Use the MethylEdge™ System with other DNA Methylation Workflow reagents to perform sensitive DNA methylation analysis on many sample types. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification technologies for detection, the MethylEdge™ Bisulfite Conversion System delivers high-quality, intact converted DNA for your experiments. MethylEdge™ Bisulfite Conversion System Features & Benefits: Effective Conversion Reagents - High-efficiency DNA conversion from as little as 100pg input DNA. Rapid Protocol - Time savings compared to other conversion systems. Intact DNA - Robust conversion of DNA with reduced DNA fragmentation. Room-Temperature, Ready-to-Use Reagents - Convenient system configuration allows room-temperature storage and minimal up-front preparation.