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Profile Lead Compounds in Own Lab

Promega Corp.
7 Jul 2010
Sarah Sarah
Marketing / Sales

Product news

Promega Corporation has partnered with SignalChem Pharmaceuticals to launch a flexible and complete kinase solution for the profiling of lead compounds against kinases representative of all major families of the kinome. Through using this single platform, scientists can easily profile lead compounds in their own laboratories in a cost-effective manner with no loss in sensitivity.

The complete solution is accomplished by combining an expanding series of unique Kinase Enzyme Systems with Promega ADP-Glo™ Kinase Assay. The Kinase Enzyme Systems, used in concert with the new universal, bioluminescent ADP-Glo Kinase Assay from Promega, provide all of the necessary tools and give drug screeners unprecedented ease to profile inhibitors against the human kinome for off-target effects.

Each Kinase System is composed of a unique protein kinase enzyme, cognate kinase substrate and enzyme buffer. All of the Kinase Enzyme Systems have been optimized and QC'd with the ADP-Glo Assay. The ADP-Glo Kinase Assay is as sensitive as radiometric kinase assays and is quickly replacing such traditional technology.

"This partnering of SignalChem's kinase reagents with the Promega ADP-Glo detection assay for kinases is an ideal fit. Now researchers can obtain all the necessary components to test protein kinase inhibition from a single source while still obtaining the level of sensitivity obtained with traditional radiometric assays." said Jasbinder Sanghera, Ph.D., President & CSO of SignalChem Pharmaceuticals, of British Columbia, Canada.

Promega is the sole distributor of Kinase Enzymes Systems from SignalChem. Each Kinase Enzyme System will be available as a stand-alone enzyme system or will be sold together with ADP-Glo Assay.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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