SelectScience
Join Free
NewsLife Sciences

Optimizing Electroporation Experimentation

Bio-Rad
11 Dec 2007

Product news

Bio-Rad Laboratories, Inc., a multinational manufacturer and distributor of life science research and clinical diagnostic products, today announced the availability of a new product guide (bulletin 5603), that describes how researchers can optimize transfections in mammalian cells using Bio-Rad’s new Gene Pulser® MXcell™ electroporation system and Gene Pulser electroporation buffer.

The four-page bulletin demonstrates how varying multiple parameters affect delivery of either siRNA or plasmid DNA into human primary fibroblasts (HPF) cells. Results of the experiments clearly demonstrate increased transfection efficiency as measured by real time Q-PCR of specific gene knockdowns or increased protein expression by luciferase assays. While many parameters can affect electroporation, with the Gene Pulser MXcell system optimization of such parameters can be accomplished in a multiplexed manner easily and rapidly in a single experiment.

Availability
Bulletin 5603 is available either from a local Bio-Rad sales office or it may be downloaded from the Bio-Rad web site via the article webpage. For more information on optimization of electroporation parameters and conditions see Bio-Rad’s technical note 5622, which can be downloaded online.

Links

Bio-RadCompany website

Tags

PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

Related articles

Optimizing Electroporation Experimentation