New Standard for High Performance Characterisation of Polymers and Proteins

12 Oct 2010
Sarah Sarah
Marketing / Sales

Product news

Dorton Analytical Ltd. announces the SEC-3010 triple detector system for the characterisation of polymers and proteins using Gel Permeation Chromatography / Size Exclusion Chromatography (GPC/SEC).

The SEC-3010 triple detector system is a complete GPC system comprising pump, autosampler, viscosity/refractive index detector, multi-angle light scattering detector and ParSEC enhanced software.

The patented design of the SEC-3010 Viscosity/Refractive Index Detector allows the simultaneous determination of the refractive index and the intrinsic viscosity of a polymer solution. With its sophisticated technology, it overcomes the disadvantages usually encountered when using coupled detectors. The asymmetrical bridge circuit allows 80 % of the sample to reach the measuring arm compared with 50 % in conventional instruments, thus increasing sensitivity by a factor of 2. The direct integration of the refractive index measuring cell in the analytical arm of the viscometer allows the determination of the intrinsic viscosity and the refractive index in exactly the same sample segment.

The Multi-Angle Light-Scattering Detector is a modern, intuitive, 7-angle detector for the comprehensive characterisation of macromolecular compounds. Its innovative technology offers the highest quality and precision in the absolute determination of molecular weight. The sample is illuminated by a very precise, temperature-controlled laser beam. The scattered light is collected by a highly-sensitive CCD detector which transmits the data to the ParSEC software.

ParSEC Enhanced provides the complete software solution for comprehensive polymer characterisation via multi-detector GPC. The software records and evaluates the data from various combinations of GPC detectors. It calculates the specific and intrinsic viscosity, the Mark-Houwink coefficients, the absolute molecular weights, Mn, Mw, Mz, Rg, the Mw/Mn structure coefficients and the branching parameters from the scattered light data. All analytical methods, raw data, calibrations, results and sample information are stored in a single database, allowing very easy archiving and retrieval of data.

The SEC-3010 system, developed by the renowned GPC company (WGE Dr Bures), offers a unique combination of comprehensive capabilities, simple, intuitive control and leading edge sensitivity all in a remarkably small laboratory footprint.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Non-Destructive TechniquesNon-destructive techniques (NDT) describes a variety of analytical techniques used to evaluate the properties of a material. Common methods include ultrasonic, magnetic-particle, liquid penetrant, radiographic, remote visual inspection (RVI), and eddy-current testing. NDT is regularly used in forensic engineering, civil engineering, mechanical engineering, electrical engineering, systems engineering, aeronautical engineering, and medicine.