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New Protein Labeling Tools for Mass Spectrometric Analysis from Thermo Fisher Scientific

27 Jun 2010
Sarah Sarah
Marketing / Sales

Product news

An expansion of Thermo Fisher Scientific's isobaric tandem mass tags product line now enables protein identification and quantitation through the selective labeling of cysteine-containing peptides from complex biological samples. The new Cysteine-Reactive Tandem Mass Tag (cysTMT) Reagents can be used in multiplex analysis of up to six different samples concurrently in an experiment.

The cysTMT Reagents label only free sulfhydryl groups on cysteine residues, in contrast to the amine-reactive TMT® Reagents. Labeled peptides are then enriched using the Thermo Scientific Immobilized Anti-TMT Antibody Resin. The cysTMT Reagents and Anti-TMT Resin are effective for reducing sample complexity, improving dynamic range and studying cysteine modifications. The cysTMTzero and cysTMTsixplex Reagents are used as isotopic “light” and “heavy” duplex tags for quantitation at the MS stage. These tags enable quantitation of protein expression changes in cell and tissue samples that might not be amenable to metabolic isotopic labeling strategies (e.g., SILAC).

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New Protein Labeling Tools for Mass Spectrometric Analysis from Thermo Fisher Scientific