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New Particles for Time-Resolved Fluorescence Applications

23 Jul 2011
bridget bridget
Laboratory Director

Product news

Thermo Fisher Scientific Inc. today announced the availability of the new Thermo Scientific Fluoro-Max Europium Chelate Fluorescent Particles designed for time-resolved fluorescence applications including clinical diagnostics, immuno/histological studies and lateral flow.

The company will showcase the products at Thermo Fisher Scientific booth #2672 during Clinical Lab Expo at AACC 2011, being held July 26 – July 28, at the Georgia World Congress Center, Atlanta, Georgia.

Available in carboxylate-modified and streptavidin-coated versions, the particles are internally dyed with europium chelate which, when excited with UV light at it maximal absorbance wavelength of 333 nm, emits long-lived fluorescence at 613 nm, creating an extremely broad Stokes Shift that prevents non-specific fluorescent interference.

"When these particles excite at 333 nm and emit at 613 nm, they do so for about 0.5 milliseconds which is a considerable length of time. In fact, this is anywhere from 10,000 to 100,000 times the lifetime of most fluorosphores. As a result, these are ideal for time-resolved fluorescence applications, such as lateral flow, nucleic acid hybridization, molecular biology, point-of-care, membrane and other applications," said Rick Galloway, director, particle technology, Thermo Fisher Scientific. "They can also be counted on to significantly lower the detection limits of fluorescent assays as they significantly reduce any background interference from relatively short-lived matrix fluorescence."

Available in 0.1, 0.2 and 0.3 µm diameter sizes, the particles are internally dyed to ensure maximum color brilliance and saturation, resulting in greater sensitivity and enhanced readability of the assay. This helps eliminate problems associated with enzyme-based assays, prevents leaching in aqueous media and provides optimum surface immunoreactivity.

For more information on the Thermo Scientific Fluoro-Max Europium Chelate Fluorescent Particles, please visit booth #2672 during AACC 2011 or go to the company article page.

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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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