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New OxyELISA™ Kit Provides Quantitative Measure of Oxidative Stress

Merck
30 Jul 2008

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Millipore Corporation, a Life Science leader providing technologies, tools and services for bioscience research and biopharmaceutical manufacturing, today announced the launch of its OxyELISA Oxidized Protein Quantitation kit, providing all reagents necessary for quantitative, high throughput detection of modified proteins in cells undergoing oxidative stress.

Oxidative stress is a cellular condition particularly relevant to aging and age-related diseases, such as Alzheimer’s disease, Parkinson’s disease, and dementia. The OxyELISA kit, by providing a fast and sensitive assay for oxidation, can help researchers, especially neuroscientists, to understand the mechanism of cellular aging.

As result of oxidative stress, oxygen free radicals and other reactive species introduce carbonyl groups into proteins in the cell. The OxyELISA kit detects these carbonyl groups, which are hallmarks of the cellular oxidative stress level. The kit uses a direct labeled monoclonal antibody that is readily detected without a secondary antibody, making the ELISA assay more specific, easier to use, and faster.

OxyELISA users can count on full technical support from Millipore, complemented by a comprehensive offering of reagents and assays for analyzing oxidative stress. Researchers can investigate superoxide dismutase activity, tyrosine nitrosylation, oxidized glutathione, and more. The OxyELISA kit complements Millipore’s more qualitative OxyBLOT™ assay, which detects oxidation of individual proteins after separation on a gel.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New OxyELISA™ Kit Provides Quantitative Measure of Oxidative Stress