New Online Tool for Automated Design of Probes and Primers for Real-Time qPCR Assays

18 Feb 2011
Sarah Sarah
Marketing / Sales

Product news

Sigma Life Science announces the release of OligoArchitect™, an online primer and probe design tool. This freely available tool offers automated design of probes and primers for real-time qPCR assays, powered by the industry standard Beacon Designer™ platform from PREMIER Biosoft, it complements Sigma Life Science’s comprehensive portfolio of oligonucleotide and qPCR products and customized oligonucleotide services.

The easy-to-use OligoArchitect software uses the latest probe design algorithms, providing results in real time and supporting templates of up to 10,000 base pairs. It also allows users to adjust various input parameters – such as homopolymer run/repeat maximum length, G/C clamp length and maximum primer pair Tm mismatch – to help optimize probe or primer design.

“After years of successful use internally by our technical support team, we wanted to offer this powerful tool directly to our customers,” said Tom Russell, Product Manager at Sigma Life Science. "We chose to use Beacon Designer because it offers best-in-class performance and is highly regarded by the research community.”

OligoArchitect currently supports both SYBR® Green I and dual-labeled probe (5’ nuclease assay) detection chemistries with additional detection chemistries and assays expected to be added later this year, making it ideally suited to key applications in traditional PCR, endpoint genotyping, gene expression analysis, genomic copy number determination, allele discrimination, SNP detection and haplotyping.

To access this tool, follow the 'Company article page' link top right of this page.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Primer Design
New Online Tool for Automated Design of Probes and Primers for Real-Time qPCR Assays