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New Microplate Provides High Recovery Extraction of Analytes from Biological Fluids

Porvair Sciences Ltd
12 Jul 2017
Abigail Berry
Administrator / Office Personnel

Product news

Porvair Sciences has announced a new easy-to-use 96-well Supported Liquid Extraction (SLE) microplate designed for high recovery extraction of analytes from biological fluids.

Using the Porvair SLE plate high analyte recovery, free of proteins and phospholipids, is routinely obtainable without the need for offline steps such as protein precipitation.

Supported Liquid Extraction is a separation technique similar to traditional liquid-liquid extraction (LLE) which uses water and an immiscible solvent to extract analytes. In SLE, the aqueous sample is immobilized on an inert support such as diatomaceous earth, and the organic phase flows through the support, eliminating problems such as emulsion formation.

Based upon the automation compatible Microlute 96-well format, the Porvair SLE plate provides fast, reproducible and economical sample clean-up when used with a simple vacuum manifold or positive pressure device. Proprietary frit technology in the plate ensures that samples will not drip or breakthrough until pressure or vacuum are applied, allowing longer dwell time in the plate wells.

The large pore size and high pore volume of the diatomaceous earth inert support, combined with a wide pH working range (1-13), allows the separation of viscous aqueous solutions such as blood, plasma and serum which may contain phospholipids and proteins. Demonstrating high recovery rates for small polar and non-polar compounds the Porvair SLE plate provides the perfect sample clean-up device for physiological fluids prior to analysis by LC/MS. The Porvair SLE plate has also been shown to be particularly helpful to environmental, food and textile labs involved in sample clean-up prior to analysis.

Find further information here.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Environmental AnalysisEnvironmental analysis describes a variety of tests that determine the effect of chemicals, processes and particulates such as persistent organic pollutants (POPs) have on the environment.BiomedicalLC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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New Microplate Provides High Recovery Extraction of Analytes from Biological Fluids