New Infrared Fluorescent Reagent Products

20 Dec 2008

Product news

LI-COR Biosciences has announced a new Infrared Fluorescent Products Guide that details a major expansion of reagent products including infrared-labeled secondary antibodies, protein labeling kits, Western blotting kits, In-Cell Western™ assay kits, Western blotting buffers, and protein stains.

New IRDye®-conjugated secondary antibodies include Donkey anti-Mouse, Donkey anti-Goat and Donkey anti-Rabbit. All antibodies are cross-adsorbed for multiplex detection and provide high specificity.

“We are pleased to expand our line of high quality infrared-dye labeled secondary antibodies. Our customers can have confidence in the quality of our infrared antibodies as they are designed and tested specifically for use with the LI-COR Odyssey® and Aerius® imaging systems,” says Neeraj Asundi, LI-COR product marketing manager.

“Our new IRDye® 800CW protein labeling kits provide researchers an excellent way to label their own antibodies and other proteins,” says Jeff Harford, LI-COR product marketing manager. “IRDye 800CW labeled proteins and antibodies are well suited for Western Blots, In-Cell Western™ Assays, and molecular imaging research.”

Western blotting and In-Cell Western™ assay kits offer the convenience of buying reagents and related products in kit form. Western blotting kits include enough secondary antibodies, blocking buffer and membranes for 10 quantitative Western blots. In-Cell Western kits include a secondary antibody, blocking buffer and protein stains (for normalization) for forty 96-well plates.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
New Infrared Fluorescent Reagent Products