New Genevac Protocol for Preparing Top Quality Lyophilized Samples

7 Nov 2013
Sarah Thomas
Associate Editor

Genevac has developed a protocol using its SuperCool 75 condenser that, in conjunction with the Genevac HT-12 High Throughput Evaporator, has been proven to set a new benchmark for enhanced evaporation and lyophilization results.

The SuperCool 75 chills to -75C and operates at higher power than any other commercially available condenser, opening the door to greatly improved results with the Genevac LyoSpeed fast lyophilization process for preparing powdered samples from HPLC fractions. Customer trials have shown that the Genevac HT-12 Evaporator / SuperCool 75 combination produces powdered lyophilization samples using the LyoSpeed methodology from even the most difficult to handle aqueous/organic solutions with a success rate as high as 96%.

Drying HPLC purification fractions, principally comprising water and acetonitrile, is a routine yet essential task in many pharmaceutical laboratories. The requirement is to dry the samples to a powder form, such that samples can be accurately weighed, easily sub-sampled and redissolved.

SuperCool 75 Condenser

Genevac

The demand for a more powerful and colder condenser has naturally arisen as the boundaries of evaporation science are pushed further and further. Users increasingly regard as critical the importance of having the right condenser for the job, with the correct power and temperature profile matched to the characteristics of the evaporator. Genevac has developed the super-powerful and super-cold SuperCool 75, in collaboration with its customers, to enhance the capabilities of the HT-12 high throughput evaporator. It has already been proven in the field to deliver enhanced evaporation and lypohilisation results.The SuperCool 75 chills to -75°C and operates at higher power than any other condenser, opening the door to greatly improved results with the Genevac LyoSpeed™ fast lyophilisation process for HPLC fractions. Customer trials show that with the most difficult to handle samples they can now achieve a success rate with LyoSpeed as high as 96%.SuperCool 75 Condenser Features: Higher power than any other condenser Chills to -75°C Gives greatly enhanced fast lyophilisation results Small footprint Developed in collaboration with customers Offers huge scope Proved in the field

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