microRNA Extraction from Biofluids Made Easy with the miRCURY™ RNA Isolation Kit - Biofluids from Exiqon

27 Nov 2012

Product news

Exiqon, a leading supplier of high-value gene expression analysis products, has announced the launch of the miRCURY™ RNA Isolation Kit - Biofluids for the extraction of small RNAs from serum, plasma, urine and other biofluids. The product is the first ever specifically optimized for such applications.

The new biofluids kit allows scientists to obtain high quality microRNA from virtually all types of biofluids in just 45 minutes. The miRCURY™ RNA Isolation Kit – Biofluids is optimized to work with Exiqon’s highly sensitive miRCURY LNA™ Universal RT microRNA PCR platform. The workflow is completed in six simple steps without the use of hazardous chemicals such as phenol and chloroform.

In a comment, Senior Vice President Sales & Marketing Henrik M. Pfundheller said: “Our customers want fully integrated research systems that allow highly robust and sensitive microRNA profiling in difficult samples such as blood and urine, and we deliver the solution with this new kit” and he added: “We have not only optimized the protocols to ensure the highest yield in the market but have also made sure that the sample does not contain any inhibitors which may jeopardize the downstream PCR analysis”.

The miRCURY™ RNA Isolation Kit – Biofluids is ideal for use with urine samples together with Exiqon’s Toxicology Focus microRNA PCR Panels, and for serum and plasma samples with the Serum/Plasma Focus microRNA PCR Panels, or indeed any of the almost 2,000 pre-validated qPCR microRNA assays available.

The miRCURY™ RNA Isolation Kit – Biofluids which can be used with a centrifuge or a vacuum manifold is provided in pack sizes of 10 and 50 units.

Exiqon offers a complete range of PCR products for microRNA analysis, which includes ready-to-use panels for genome-wide screening of human, mouse and rat microRNAs, numerous focus panels, the fully flexible Pick & Mix panels, spike-in kits, cDNA synthesis and PCR Master Mix kits, as well as unique qPCR data analysis software. Customers that have identified novel microRNAs can take advantage of Exiqon’s proprietary LNA™ chemistry and unique design algorithm through easy-to-use online tool for high quality design of custom microRNA qPCR assays.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.UrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses. PlasmamiRNA